Pyramiding of resistance alleles to grape powdery mildew assisted by molecular markers

Disease resistance gene pyramiding is a widely used strategy to enhance durability. Marker-assisted selection (MAS) was applied pyramide the alleles Run1 and Ren3, which confer against grape powdery mildew (Erysiphe necator). Two F1 full-sibs carrying Ren3 in heterozygosity were selfed develop breeding populations analysis. From 637 genotyped plants, 313 (50.6%) had pyramided. Seven (1.1%) of them exhibited two homozygosity. Plants without any highest disease severity ( = 7.3), while ones with allele both homozygosity highly resistant 1.5). Similar level observed plants containing pyramided 1.3). are important genetic resources for programs Brazil.